Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells. Transfusion 47, 14361446. 10.1111/j.1537-2995.2007.01220.x [Google Scholar] Schallmoser K., Strunk D. (2013). Generation of a pool of human platelet lysate and efficient use in cell culture.
Evaluation of human platelet lysate versus fetal bovine Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells HATIM HEMEDA1, BERND GIEBEL2 & WOLFGANG WAGNER1 1Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany, and 2Institute for Transfusion Medicine, University Hospital Essen, Essen, Germany
Feb 01, 2014 · Human platelet lysate has several advantages and disadvantages in comparison to FBS :i) many studies indicated that hPL supports culture expansion of MSCs even better than FBS, HS, PRP or tPRP 33, 41, 48, 53. Particularly, proliferation of adipose tissuederived MSCs is largely increased with hPL 58, 59, 60. This growth advantage was less
Frontiers Comparative Analysis of Mesenchymal Stem Cell Jan 15, 2021 · Human platelet lysate (hPL) appears to be a valuable alternative to widely used fetal calf serum (FCS) since no ethical issues are associated with its harvest, it contains a high concentration of growth factors and cytokines and it can be produced from expired platelet concentrate. Serum and xeno-free, chemically defined, no-plate-coating
GEO Accession viewerHuman serum and heparin-free platelet lysate as appropriate xeno-free alternatives for production of human MuStem cell batches:Organism:Homo sapiens:Experiment type:Eion profiling by high throughput sequencing:Summary
This article reviews how human platelet lysate can be used in place of FBS to expand human cells ex vivo:A pathogen-free and standardized xeno-free supplement of growth media is required for the
Human Alternatives to Fetal Bovine Serum for the Expansion Jun 04, 2009 · Charlotte Saury, Aurélie Lardenois, Cindy Schleder, Isabelle Leroux, Blandine Lieubeau, Laurent David, Marine Charrier, Laëtitia Guével, Sabrina Viau, Bruno Delorme, Karl Rouger, Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches, Stem Cell Research & Therapy, 10
Human FGF basic/FGF2/bFGF Quantikine ELISA Kit DFB50:Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches Authors:C Saury, A Lardenois, C Schleder, I Leroux, B Lieubeau, L David, M Charrier, L Guével, S Viau, B Delorme, K
Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches Authors:C Saury, A Lardenois, C Schleder, I Leroux, B Lieubeau, L David, M Charrier, L Guével, S Viau, B Delorme, K Rouger Stem Cell
Human Platelet Lysate (HPL) - Life Science ProductionHuman Platelet Lysate (hPL) is a cell culture growth supplements obtained from human platelets. HPL contains abundant growth factors and cytokines necessary for cell growth and proliferation. Replacing foetal bovine serum (FBS) with hPL allows for expansion and potential clinical grade production of functional mesenchymal stromal cells by removing the risk of xenogeneic
Human Platelet Lysate (HPL) - more details - Life Science Human Platelet Lysate can be used as a replacement for traditional foetal bovine supplemented cell culture medium. HPL is a suitable alternative for use in R&D and production/ manufacturing processes. Improves the proliferation of Mesenchymal Stromal Cells (MSCs) compared to FBS without loss of phenotype.
Nov 27, 2019 · Saury C et al 2018 Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches Stem Cell Res. Ther. 9 128. Crossref Google Scholar
Human platelet lysate:Replacing fetal bovine serum as a Human platelet lysate can replace fetal bovine serum (FBS) for xeno-free ex vivo expansion of mesenchymal stromal cells (MSCs), but pooling of platelet concentrates (PCs) increases risks of pathogen transmission. We evaluated the feasibility of performing nanofiltration of platelet lysates and determined the impact on expansion of bone marrowderived MSCs.
Non-pooled Human Platelet Lysate:A Potential Serum Over the past few years, many research groups have worked on the development of various serum replacements, which are referred to as serum-free, xeno-free or chemically defined supplements. 3,6,14 A number of studies have shown that serum supplements from various human origins have the potential to replace FBS in cell culture
In both research and clinical applications, human platelet lysate is often used as an alternative to animal sera such as FBS, in order to minimize the risk of xenogenic immune reactions or transmitting prions and zoonotic diseases5. PLTGold® Human Platelet Lysate is a xeno-free, animal serum-free supplement, proven to showGEO Accession viewerThe aim of the current study was to evaluate the use of two xeno-free blood derivatives corresponding to human serum (HS) and human platelet lysate (hPL) as alternatives to controverted but until now used fetal bovine serum (FBS) for isolation and expansion of human MuStem (hMuStem) cells.